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KMID : 0380020100250040351
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Korean Journal of Biotechnology and Bioengineering
2010 Volume.25 No. 4 p.351 ~ p.356
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Ethanol Production with Glucose/Xylose Mixture by Immobilized Pichia stipitis
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Shin Hyun-Seok
Kang Seong-Woo
Lee Sang-Jun
Jang Eun-Ji
Suh Young-Woong
Kim Seung-Wook
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Abstract
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To increase the production of ethanol by using sugar from lignocellulosic biomass, pentose and hexose have to be fermented simultaneously by yeast. The effects of mixed sugar and nitrogen on ethanol production by immobilized Pichia stipitis KCCM 12009 were investigated. When optimal mixed sugar and nitrogen concentration were 5% (Glucose/Xylose = 3：1) and 1%, respectively, ethanol concentration produced by immobilized P. stipitis was 19-20 g/L. In repeated fed-batch by immobilized P. stipitis, all glucose was consumed very quickly at 1-3% mixed sugar concentration. But, xylose consumption was decreased as the mixed sugar concentration increased. Also, ethanol (5.6 g/L) was stably produced and ethanol production rate was 0.13 g/L ? h in immobilized cell reactor (ICR) with 1% mixed sugar (Glucose/Xylose = 3：1) as feeding media.
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KEYWORD
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Ethanol, Glucose/xylose mixture, Pichia stipitis, Immobilized cell reactor (ICR)
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